| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ritchie, Matthew J (9)
|
UNAT |
38.69 |
37.40 |
-1.29 |
| 2 |
Delos Santos, Joshua B (8)
|
NCGFSC |
40.32 |
37.54 |
-2.78 |
| 3 |
Clayton, Thomas J (9)
|
NCGFSC |
38.74 |
38.76 |
0.02 |
| 4 |
Torsone, Bryce G (9)
|
NCGFSC |
43.11 |
42.97 |
-0.14 |
| 5 |
Earl, Tyler K (10)
|
UNAT |
42.29 |
43.81 |
1.52 |
| 6 |
Aberant, Thomas A (10)
|
NCGFSC |
43.41 |
44.59 |
1.18 |
| 7 |
Payne, Charlie W (9)
|
NCGFSC |
42.94 |
45.33 |
2.39 |
| 8 |
Johnson, Connor R (10)
|
NCGOLD |
47.56 |
45.96 |
-1.60 |
| 9 |
Jones, Noah C (9)
|
NCYBAC |
43.87 |
46.89 |
3.02 |
| 10 |
Pendleton, Zackary A (10)
|
NCGFSC |
53.99 |
50.21 |
-3.78 |
| 11 |
Gribbin, Harry G (9)
|
NCGOLD |
1:02.11 |
52.89 |
-9.22 |
| 12 |
Singletary, Cohen P (10)
|
NCLTNC |
51.99 |
56.07 |
4.08 |
| 13 |
Rodeffer, Matthew J (9)
|
NCLTNC |
|
58.14 |
- |
| 14 |
Prakash, Anirudh (9)
|
NCWBST |
1:06.59 |
DQ |
- |
| 15 |
Lee, Christopher W (9)
|
NCRMY |
|
1:15.83 |
- |
|
Coetzer, Eli C (10)
|
NCWBST |
55.06 |
DQ |
- |